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Quantitative Forster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra

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dc.contributor.authorMustafa, Sanam
dc.contributor.authorHannagan, John
dc.contributor.authorRigby, Paul
dc.contributor.authorPfleger, Kevin D. G.
dc.contributor.authorCorry, Ben
dc.date.accessioned2015-12-13T22:39:21Z
dc.date.issued2013
dc.date.updated2016-02-24T09:30:05Z
dc.description.abstractAccurate quantification of Förster resonance energy transfer (FRET) using intensity-based methods is difficult due to the overlap of fluorophore excitation and emission spectra. Consequently, mechanisms are required to remove bleedthrough of the donor em
dc.identifier.issn1083-3668
dc.identifier.urihttp://hdl.handle.net/1885/77752
dc.publisherSPIE - The International Society for Optical Engineering
dc.rightsAuthor/s retain copyrighten_AU
dc.sourceJournal of Biomedical Optics
dc.subjectKeywords: fluorescent dye; photoprotein; algorithm; article; cell strain HEK293; chemistry; fluorescence microscopy; fluorescence resonance energy transfer; genetics; human; light related phenomena; metabolism; methodology; statistics; Algorithms; Fluorescence Reso
dc.titleQuantitative Forster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra
dc.typeJournal article
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue2
local.bibliographicCitation.lastpage14)
local.bibliographicCitation.startpage026024(1
local.contributor.affiliationMustafa, Sanam, Western Australian Insitutue for Medical Research
local.contributor.affiliationHannagan, John, The University of Western Australia
local.contributor.affiliationRigby, Paul, The University of Western Australian
local.contributor.affiliationPfleger, Kevin D. G., University of Western Australia
local.contributor.affiliationCorry, Ben, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidCorry, Ben, u9719358
local.description.notesImported from ARIES
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.absfor060112 - Structural Biology (incl. Macromolecular Modelling)
local.identifier.absfor029901 - Biological Physics
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo970111 - Expanding Knowledge in the Medical and Health Sciences
local.identifier.absseo920111 - Nervous System and Disorders
local.identifier.ariespublicationf5625xPUB6544
local.identifier.citationvolume18
local.identifier.doi10.1117/1.JBO.18.2.026024
local.identifier.scopusID2-s2.0-84883570466
local.identifier.thomsonID000315159900038
local.type.statusPublished Version

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