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Quantitative Forster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra

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Date

2013

Authors

Mustafa, Sanam
Hannagan, John
Rigby, Paul
Pfleger, Kevin D. G.
Corry, Ben

Journal Title

Journal ISSN

Volume Title

Publisher

SPIE - The International Society for Optical Engineering

Abstract

Accurate quantification of Förster resonance energy transfer (FRET) using intensity-based methods is difficult due to the overlap of fluorophore excitation and emission spectra. Consequently, mechanisms are required to remove bleedthrough of the donor em

Description

Keywords

Keywords: fluorescent dye; photoprotein; algorithm; article; cell strain HEK293; chemistry; fluorescence microscopy; fluorescence resonance energy transfer; genetics; human; light related phenomena; metabolism; methodology; statistics; Algorithms; Fluorescence Reso

Citation

URI

http://hdl.handle.net/1885/77752

Collections

ANU Research Publications

Source

Journal of Biomedical Optics

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

DOI

10.1117/1.JBO.18.2.026024

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Description
01_Mustafa_Quantitative_Forster_resonance_2013.pdf (5.78 MB)

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