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Achieving sub-micrometer imaging resolution in PDMS soft lithography devices using modified inverted selective plane illumination microscopy

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dc.contributor.authorXu, Tao
dc.contributor.authorLim, Ying Jun
dc.contributor.authorZheng, Yujie
dc.contributor.authorGardiner, Elizabeth
dc.contributor.authorJung, M.S.
dc.contributor.authorGaus, Katharina
dc.contributor.authorLee, W M Steve
dc.coverage.spatialVirtual, Online
dc.date.accessioned2024-02-16T00:57:56Z
dc.date.created4-9 October 2020
dc.date.issued2020
dc.date.updated2022-05-08T08:16:09Z
dc.description.abstractWe demonstrated light sheet fluorescence imaging at sub-micrometer resolution of biological samples enclosed in PDMS soft lithography devices. This was achieved by minimizing the optical distortion induced at the external PDMS device interface using a sucrose solution with refractive index (RI) matched to the PDMS. A commercially available inverted Selective Plane Illumination Microscopy (iSPIM) was modified with a single mode fiber (SMF) and an electrically tunable lens (ETL) module to facilitate RI matching and compensate for sucrose solution induced axial focal shift. We termed this protocol as the modified iSPIM (m-iSPIM).en_AU
dc.description.sponsorshipThis work was supported by grants Australian Research Council (DP200100364, DP190100039, DE160100843) and ANU Major Equipment grant (15MEC36, 16MEC26) and additional funds from ARC Centre of Excellence Translational Photosynthesis.en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.isbn978-173341901-7en_AU
dc.identifier.urihttp://hdl.handle.net/1885/313644
dc.language.isoen_AUen_AU
dc.publisherChemical and Biological Microsystems Societyen_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP200100364en_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP190100039en_AU
dc.relationhttp://purl.org/au-research/grants/arc/DE160100843en_AU
dc.relation.ispartofseries24th International Conference on Miniaturized Systems for Chemistry and Life Sciencesen_AU
dc.rights© Chemical and Biological Microsystems Societyen_AU
dc.titleAchieving sub-micrometer imaging resolution in PDMS soft lithography devices using modified inverted selective plane illumination microscopyen_AU
dc.typeConference paperen_AU
local.bibliographicCitation.lastpage560en_AU
local.bibliographicCitation.startpage558en_AU
local.contributor.affiliationXu, Tao, College of Engineering and Computer Science, ANUen_AU
local.contributor.affiliationLim, Ying Jun, College of Health and Medicine, ANUen_AU
local.contributor.affiliationZheng, Yujie, College of Engineering and Computer Science, ANUen_AU
local.contributor.affiliationGardiner, Elizabeth, College of Health and Medicine, ANUen_AU
local.contributor.affiliationJung, M.S., EMBL Australia Node in Single Molecule Scienceen_AU
local.contributor.affiliationGaus, Katharina, University of New South Walesen_AU
local.contributor.affiliationLee, Steve, College of Health and Medicine, ANUen_AU
local.contributor.authoruidXu, Tao, u5527268en_AU
local.contributor.authoruidLim, Ying Jun, u4660484en_AU
local.contributor.authoruidZheng, Yujie, u5173962en_AU
local.contributor.authoruidGardiner, Elizabeth, u1023050en_AU
local.contributor.authoruidLee, Steve, u5343203en_AU
local.description.embargo2099-12-31
local.description.notesImported from ARIESen_AU
local.description.refereedYes
local.identifier.absfor400304 - Biomedical imagingen_AU
local.identifier.absfor401809 - Nanophotonicsen_AU
local.identifier.absseo280110 - Expanding knowledge in engineeringen_AU
local.identifier.ariespublicationa383154xPUB16920en_AU
local.identifier.scopusID2-s2.0-85098265188
local.type.statusPublished Versionen_AU

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