Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase

Date

2000

Authors

Walker, Ian
Easton, Christopher
Ollis, David

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry

Abstract

Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.

Description

Keywords

Keywords: hydrolase; isomerase; lactone; article; catalysis; enzyme active site; enzyme activity; enzyme modification; hydrolysis; isomerism; site directed mutagenesis

Citation

Source

Chemical Communications

Type

Journal article

Book Title

Entity type

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