Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase
| dc.contributor.author | Walker, Ian | |
| dc.contributor.author | Easton, Christopher | |
| dc.contributor.author | Ollis, David | |
| dc.date.accessioned | 2015-12-13T23:18:05Z | |
| dc.date.available | 2015-12-13T23:18:05Z | |
| dc.date.issued | 2000 | |
| dc.date.updated | 2015-12-12T08:55:25Z | |
| dc.description.abstract | Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase. | |
| dc.identifier.issn | 1359-7345 | |
| dc.identifier.uri | http://hdl.handle.net/1885/90004 | |
| dc.publisher | Royal Society of Chemistry | |
| dc.source | Chemical Communications | |
| dc.subject | Keywords: hydrolase; isomerase; lactone; article; catalysis; enzyme active site; enzyme activity; enzyme modification; hydrolysis; isomerism; site directed mutagenesis | |
| dc.title | Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase | |
| dc.type | Journal article | |
| local.bibliographicCitation.lastpage | 672 | |
| local.bibliographicCitation.startpage | 671 | |
| local.contributor.affiliation | Walker, Ian, College of Physical and Mathematical Sciences, ANU | |
| local.contributor.affiliation | Easton, Christopher, College of Physical and Mathematical Sciences, ANU | |
| local.contributor.affiliation | Ollis, David, College of Physical and Mathematical Sciences, ANU | |
| local.contributor.authoruid | Walker, Ian, u4044638 | |
| local.contributor.authoruid | Easton, Christopher, u9500570 | |
| local.contributor.authoruid | Ollis, David, u9200080 | |
| local.description.notes | Imported from ARIES | |
| local.description.refereed | Yes | |
| local.identifier.absfor | 030499 - Medicinal and Biomolecular Chemistry not elsewhere classified | |
| local.identifier.ariespublication | MigratedxPub20261 | |
| local.identifier.citationvolume | 8 | |
| local.identifier.scopusID | 2-s2.0-0034696912 | |
| local.type.status | Published Version |