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Multiple-site labeling of proteins with unnatural amino acids

dc.contributor.authorLoscha, Karinen_AU
dc.contributor.authorHerlt, Anthonyen_AU
dc.contributor.authorQi, Ruhuen_AU
dc.contributor.authorOzawa, Kiyoshien_AU
dc.contributor.authorOtting, Gottfrieden_AU
dc.contributor.authorHuber, Thomasen_AU
dc.date.accessioned2015-12-10T22:58:23Z
dc.date.issued2012
dc.date.updated2016-02-24T10:24:49Z
dc.description.abstractA cell-free protein synthesis system from which the release factor RF1 has been selectively removed enables the facile incorporation of unnatural amino acids into proteins at difficult and multiple sites by optimized use of orthogonal tRNA/aminoacyl-tRNA
dc.identifier.issn1433-7851
dc.identifier.urihttp://hdl.handle.net/1885/60833
dc.publisherWiley-VCH Verlag GMBH
dc.sourceAngewandte Chemie International Edition
dc.subjectKeywords: Cell free protein synthesis; Number of samples; Optimized use; protein modifications; Release factors; Resonance assignments; Synthetases; termination factor RF1; Trifluoromethyl; Amino acids; Nuclear magnetic resonance spectroscopy; Proteins; amino acid; cell-free protein synthesis; NMR spectroscopy; protein modifications; termination factor RF1; unnatural amino acids
dc.titleMultiple-site labeling of proteins with unnatural amino acids
dc.typeJournal article
local.bibliographicCitation.issue9
local.bibliographicCitation.lastpage2246
local.bibliographicCitation.startpage2243
local.contributor.affiliationLoscha, Karin, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationHerlt, Anthony, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationQi, Ruhu, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationHuber, Thomas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOzawa, Kiyoshi, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.contributor.authoruidLoscha, Karin, u3331903
local.contributor.authoruidHerlt, Anthony, u7100439
local.contributor.authoruidQi, Ruhu, u4005714
local.contributor.authoruidHuber, Thomas, u9512183
local.contributor.authoruidOzawa, Kiyoshi, u4050581
local.contributor.authoruidOtting, Gottfried, u4046684
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor030402 - Biomolecular Modelling and Design
local.identifier.absfor030403 - Characterisation of Biological Macromolecules
local.identifier.absfor030406 - Proteins and Peptides
local.identifier.absseo970103 - Expanding Knowledge in the Chemical Sciences
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.ariespublicationu4005981xPUB567
local.identifier.citationvolume51
local.identifier.doi10.1002/anie.201108275
local.identifier.scopusID2-s2.0-84857508460
local.identifier.thomsonID000300691900047
local.type.statusPublished Version

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