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Multiple-site labeling of proteins with unnatural amino acids

Date

Authors

Loscha, Karin

Herlt, Anthony

Qi, Ruhu

Ozawa, Kiyoshi

Otting, Gottfried

Huber, Thomas

Publisher

Wiley-VCH Verlag GMBH

Abstract

A cell-free protein synthesis system from which the release factor RF1 has been selectively removed enables the facile incorporation of unnatural amino acids into proteins at difficult and multiple sites by optimized use of orthogonal tRNA/aminoacyl-tRNA

Keywords

Keywords: Cell free protein synthesis; Number of samples; Optimized use; protein modifications; Release factors; Resonance assignments; Synthetases; termination factor RF1; Trifluoromethyl; Amino acids; Nuclear magnetic resonance spectroscopy; Proteins; amino acid; cell-free protein synthesis; NMR spectroscopy; protein modifications; termination factor RF1; unnatural amino acids

URI

http://hdl.handle.net/1885/60833

Collections

ANU Research Publications

Source

Angewandte Chemie International Edition

Type

Journal article

DOI

10.1002/anie.201108275

Restricted until

2037-12-31

Downloads

File

Description

01_Loscha_Multiple-site_labeling_of_2012.pdf (612.62 KB)