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Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains

Date

Authors

Shaw, Denis

Shultz, Brandon

Ramsland, Paul

Edmundson, Allen

Publisher

John Wiley & Sons Inc

Abstract

The VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemi

Keywords

Keywords: dodecyl sulfate sodium; immunoglobulin G; immunoglobulin heavy chain; monoclonal antibody; Bence Jones protein; immunoglobulin; immunoglobulin M; myeloma immunoglobulins; paraprotein; amino acid sequence; polyacrylamide gel electrophoresis; protein degrad Antibody crystallography; Human monoclonal antibodies; Insoluble peptides; Protein sequencing

URI

http://hdl.handle.net/1885/94297

Collections

ANU Research Publications

Source

Journal of Molecular Recognition

Type

Journal article

DOI

10.1002/jmr.596