Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains
Date
2002
Authors
Shaw, Denis
Shultz, Brandon
Ramsland, Paul
Edmundson, Allen
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons Inc
Abstract
The VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemi
Description
Keywords
Keywords: dodecyl sulfate sodium; immunoglobulin G; immunoglobulin heavy chain; monoclonal antibody; Bence Jones protein; immunoglobulin; immunoglobulin M; myeloma immunoglobulins; paraprotein; amino acid sequence; polyacrylamide gel electrophoresis; protein degrad Antibody crystallography; Human monoclonal antibodies; Insoluble peptides; Protein sequencing
Citation
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Source
Journal of Molecular Recognition
Type
Journal article