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Plasmodium falciparum culture: The benefits of shaking

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dc.contributor.authorAllen, Richard
dc.contributor.authorKirk, Kiaran
dc.date.accessioned2015-12-07T22:27:44Z
dc.date.issued2010
dc.date.updated2016-02-24T11:41:25Z
dc.description.abstractDespite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced.
dc.identifier.issn0166-6851
dc.identifier.urihttp://hdl.handle.net/1885/22029
dc.publisherElsevier
dc.sourceMolecular and Biochemical Parasitology
dc.subjectKeywords: sorbitol; article; cell culture; cell suspension; cell synchronization; controlled study; DNA modification; genetic transfection; malaria falciparum; nonhuman; parasite cultivation; Plasmodium falciparum; population dynamics; prevalence; priority journal; Culture; Multiply infected erythrocytes; Shaking; Static; Synchronisation; Transfected parasites
dc.titlePlasmodium falciparum culture: The benefits of shaking
dc.typeJournal article
local.bibliographicCitation.issue2010
local.bibliographicCitation.lastpage65
local.bibliographicCitation.startpage63
local.contributor.affiliationAllen, Richard, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKirk, Kiaran, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidAllen, Richard, u9806959
local.contributor.authoruidKirk, Kiaran, u9608579
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.absseo920109 - Infectious Diseases
local.identifier.ariespublicationu8611701xPUB19
local.identifier.citationvolume169
local.identifier.doi10.1016/j.molbiopara.2009.09.005
local.identifier.scopusID2-s2.0-71549139434
local.identifier.thomsonID000272644900009
local.type.statusPublished Version

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