Plasmodium falciparum culture: The benefits of shaking

Date

2010

Authors

Allen, Richard
Kirk, Kiaran

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Despite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced.

Description

Keywords

Keywords: sorbitol; article; cell culture; cell suspension; cell synchronization; controlled study; DNA modification; genetic transfection; malaria falciparum; nonhuman; parasite cultivation; Plasmodium falciparum; population dynamics; prevalence; priority journal; Culture; Multiply infected erythrocytes; Shaking; Static; Synchronisation; Transfected parasites

Citation

Source

Molecular and Biochemical Parasitology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/j.molbiopara.2009.09.005

Restricted until

2037-12-31