Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy
Date
2011
Authors
Swarbrick, James
Ung, Phuc
Su, Xun-Cheng
Maleckis, Ansis
Chhabra, Sandeep
Graham, Bim
Otting, Gottfried
Huber, Thomas
Journal Title
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Volume Title
Publisher
Royal Society of Chemistry
Abstract
Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudoconta
Description
Keywords
Keywords: edetic acid; lanthanide; nitrilotriacetic acid; alpha helix; article; binding affinity; cross coupling reaction; ligand binding; nuclear magnetic resonance spectroscopy; protein binding; protein engineering; protein motif; protein protein interaction; Ami
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Source
Chemical Communications
Type
Journal article
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2037-12-31