The use of genomewide ENU mutagenesis screens to unravel complex mammalian traits: identifying genes that regulate organ-specific and systemic autoimmunity
Date
2006
Authors
Hoyne, Gerard
Goodnow, Christopher
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Publisher
Munksgaard International Publishers
Abstract
T-cell development is perhaps one of the best understood processes of mammalian cell differentiation, as many of the genes and pathways have been identified. By contrast, relatively little is known about the genes and pathways involved in immunological tolerance to self-antigens. Here, we describe the challenges associated with a genomewide screen designed at identifying new immune regulatory genes that uses a model of organ-specific autoimmunity leading to type 1 diabetes. The successful propagation and identification of the new gene variants will shed light on the various developmental checkpoints in lymphocyte development that are crucial for establishing tolerance to self-antigens.
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Keywords
Keywords: ethylnitrosourea; lymphocyte antigen receptor; autoimmunity; cell maturation; correlation analysis; diagnostic value; gene deletion; gene identification; gene library; gene mapping; gene sequence; genetic screening; genetic susceptibility; genetic trait;
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Source
Immunological Reviews
Type
Journal article
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Restricted until
2037-12-31
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