The enigmatic genome of Chara australis virus
Date
2011
Authors
Gibbs, Adrian
Torronen, Marjolein
Mackenzie, Anne
Wood, Jeffrey
Armstrong, John
Kondo, Hideki
Tamada, Tetsuo
Keese, Paul
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Society for General Microbiology
Abstract
Most of the genomic sequence of Chara australis virus (CAV), previously called Chara corallina virus, has been determined. It is a ssRNA molecule of 9065 nt with at least four ORFs. At its 59 end is an ORF encoding a protein of 227 kDa, distantly homologous to the multifunctional replicases of benyviruses and rubiviruses. Next is an ORF encoding a protein of 44 kDa, homologous to the helicases of pestiviruses. The third ORF encodes an unmatched protein of 38 kDa that is probably a movement protein. The fourth and 39-terminal ORF encodes a protein of 17.7 kDa homologous to the coat proteins of tobamoviruses. The short methyltransferase region of the CAV replicase matches only the C-terminal motif of benyvirus methyltransferases. This and other clues indicate that approximately 11% and 2% of the 59 and 39 termini of the complete CAV genome, respectively, are missing from the sequence. The aligned amino acid sequences of the CAV proteins and their nearest homologues contain many gaps but relationships inferred from them were little affected by removal of these gaps. Sequence comparisons show that three of the CAV genes may have diverged from the most closely related genes of other viruses 250-450 million years ago, and the sister relationship between the genes of CAV and those of benyviruses and tobamoviruses, mirroring the ancient sister relationship between charophytes (i.e. the algal host of CAV) and embryophytes (i.e. the plant hosts of tobamoviruses and benyviruses), is congruent with this possibility.
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Keywords
Keywords: coat protein; helicase; methyltransferase; orf 10 protein; orf 2 protein; orf 7 protein; orf 8 protein; unclassified drug; virus protein; amino acid sequence; article; chara australis; gene sequence; genetic analysis; nonhuman; nucleotide sequence; Pestiv
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Journal of General Virology
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Journal article
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2037-12-31
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