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In situ deprotection and incorporation of unnatural amino acids during cell-free protein synthesis

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dc.contributor.authorArthur, Isaac
dc.contributor.authorHennessy, James E
dc.contributor.authorPadmakshan, Dharshana
dc.contributor.authorStigers, Dannon
dc.contributor.authorLesturgez, Stephanie
dc.contributor.authorFraser, Samuel
dc.contributor.authorLiutkus, Mantas
dc.contributor.authorOakeshott , John
dc.contributor.authorEaston, Christopher
dc.contributor.authorOtting, Gottfried
dc.date.accessioned2015-12-13T22:23:19Z
dc.date.issued2013
dc.date.updated2016-02-24T09:10:29Z
dc.description.abstractThe S30 extract from E. coli BL21 Star (DE3) used for cell-free protein synthesis removes a wide range of α-amino acid protecting groups by cleaving α-carboxyl hydrazides; methyl, benzyl, tert-butyl, and adamantyl esters; tert-butyl and adamantyl carbox
dc.identifier.issn0947-6539
dc.identifier.urihttp://hdl.handle.net/1885/72720
dc.publisherWiley-VCH Verlag GMBH
dc.sourceChemistry, A European Journal
dc.subjectKeywords: Cell free protein synthesis; Cell-free; Enzyme catalysis; Free amino acids; Protecting group; Protein expressions; Standard conditions; Unnatural amino acids; Amino acids; Escherichia coli; Esterification; Esters; Machinery; Proteins; Biosynthesis amino acids; cell-free synthesis; enzyme catalysis; protecting groups; protein expression
dc.titleIn situ deprotection and incorporation of unnatural amino acids during cell-free protein synthesis
dc.typeJournal article
local.bibliographicCitation.issue21
local.bibliographicCitation.lastpage6830
local.bibliographicCitation.startpage6824
local.contributor.affiliationArthur, Isaac, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationHennessy, James E, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationPadmakshan, Dharshana, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationStigers, Dannon, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationLesturgez, Stephanie, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationFraser, Samuel, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationLiutkus, Mantas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOakeshott , John, CSIRO Ecosystems Science
local.contributor.affiliationEaston, Christopher, College of Physical and Mathematical Sciences, ANU
local.contributor.authoruidArthur, Isaac, u4297659
local.contributor.authoruidHennessy, James E, u4277962
local.contributor.authoruidPadmakshan, Dharshana, u4139883
local.contributor.authoruidStigers, Dannon, u4634162
local.contributor.authoruidLesturgez, Stephanie, u5047042
local.contributor.authoruidFraser, Samuel, u4813630
local.contributor.authoruidLiutkus, Mantas, u4972333
local.contributor.authoruidOtting, Gottfried, u4046684
local.contributor.authoruidEaston, Christopher, u9500570
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor030503 - Organic Chemical Synthesis
local.identifier.absseo970103 - Expanding Knowledge in the Chemical Sciences
local.identifier.ariespublicationf5625xPUB3396
local.identifier.citationvolume19
local.identifier.doi10.1002/chem.201203923
local.identifier.scopusID2-s2.0-84877778114
local.identifier.thomsonID000318923800034
local.type.statusPublished Version

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