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In situ deprotection and incorporation of unnatural amino acids during cell-free protein synthesis

Date

2013

Authors

Arthur, Isaac
Hennessy, James E
Padmakshan, Dharshana
Stigers, Dannon
Lesturgez, Stephanie
Fraser, Samuel
Liutkus, Mantas
Oakeshott , John
Easton, Christopher
Otting, Gottfried

Journal Title

Journal ISSN

Volume Title

Publisher

Wiley-VCH Verlag GMBH

Abstract

The S30 extract from E. coli BL21 Star (DE3) used for cell-free protein synthesis removes a wide range of α-amino acid protecting groups by cleaving α-carboxyl hydrazides; methyl, benzyl, tert-butyl, and adamantyl esters; tert-butyl and adamantyl carbox

Description

Keywords

Keywords: Cell free protein synthesis; Cell-free; Enzyme catalysis; Free amino acids; Protecting group; Protein expressions; Standard conditions; Unnatural amino acids; Amino acids; Escherichia coli; Esterification; Esters; Machinery; Proteins; Biosynthesis amino acids; cell-free synthesis; enzyme catalysis; protecting groups; protein expression

Citation

Source

Chemistry, A European Journal

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1002/chem.201203923

Restricted until

2037-12-31