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Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

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dc.contributor.authorWu, Peter
dc.contributor.authorOzawa, Kiyoshi
dc.contributor.authorLim, Siew P
dc.contributor.authorVasudevan, Subhash G.
dc.contributor.authorDixon, Nicholas Edward
dc.contributor.authorOtting, Gottfried
dc.date.accessioned2015-12-07T22:54:19Z
dc.date.issued2007
dc.date.updated2015-12-07T12:48:42Z
dc.description.abstractQuick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).
dc.identifier.issn1433-7851
dc.identifier.urihttp://hdl.handle.net/1885/28139
dc.publisherWiley-VCH Verlag GMBH
dc.sourceAngewandte Chemie International Edition
dc.subjectKeywords: Cell culture; Cyclization; Enzyme kinetics; Proteins; Reaction kinetics; Transcription; Cell-free protein synthesis; High-throughput methods; Polymerase chain reaction; DNA; cell extract; DNA; article; cell free system; cytology; Escherichia coli; genetic Cell-free protein synthesis; High-throughput methods; NMR spectroscopy; Polymerase chain reaction
dc.titleCell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies
dc.typeJournal article
local.bibliographicCitation.issue18
local.bibliographicCitation.lastpage3358
local.bibliographicCitation.startpage3356
local.contributor.affiliationWu, Peter, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOzawa, Kiyoshi, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationLim, Siew P, Novartis Institute for Tropical Diseases Pty Ltd
local.contributor.affiliationVasudevan, Subhash G., Novartis Institute for Tropical Diseases Pty Ltd
local.contributor.affiliationDixon, Nicholas Edward, University of Wollongong
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.contributor.authoruidWu, Peter, u4051785
local.contributor.authoruidOzawa, Kiyoshi, u4050581
local.contributor.authoruidOtting, Gottfried, u4046684
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor069999 - Biological Sciences not elsewhere classified
local.identifier.ariespublicationu4005981xPUB56
local.identifier.citationvolume46
local.identifier.doi10.1002/anie.200605237
local.identifier.scopusID2-s2.0-34250826547
local.type.statusPublished Version

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