Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies
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Wu, Peter
Ozawa, Kiyoshi
Lim, Siew P
Vasudevan, Subhash G.
Dixon, Nicholas Edward
Otting, Gottfried
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Wiley-VCH Verlag GMBH
Abstract
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).
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Angewandte Chemie International Edition
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2037-12-31
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