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PEA-CLARITY: 3D molecular imaging of whole plant organs

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dc.contributor.authorPalmer, William M
dc.contributor.authorMartin, Antony P
dc.contributor.authorFlynn, Jamie R
dc.contributor.authorReed, Stephanie L.
dc.contributor.authorWhite, Rosemary Gillian
dc.contributor.authorFurbank, Robert
dc.contributor.authorGrof, C.P.L.
dc.date.accessioned2016-06-13T23:26:17Z
dc.date.issued2015
dc.date.updated2016-06-10T08:16:25Z
dc.description.abstractHere we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating protein localisation of intact tissue in 3D whilst retaining cellular structure.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1885/102542
dc.publisherNature Publishing Group
dc.rightsAuthor/s retain copyrighten_AU
dc.sourceScientific Reports
dc.titlePEA-CLARITY: 3D molecular imaging of whole plant organs
dc.typeJournal article
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue5:13492
local.contributor.affiliationPalmer, William M , University of Newcastle
local.contributor.affiliationMartin, Antony P, University of Newcastle
local.contributor.affiliationFlynn, Jamie R, University of Newcastle
local.contributor.affiliationReed, Stephanie L., University of Newcastle
local.contributor.affiliationWhite, Rosemary Gillian, CSIRO Division of Plant Industry
local.contributor.affiliationFurbank, Robert, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGrof, C.P.L., CSIRO Plant Industry
local.contributor.authoruidFurbank, Robert, u1572217
local.description.notesImported from ARIES
local.identifier.absfor060705 - Plant Physiology
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.ariespublicationU3488905xPUB6172
local.identifier.citationvolume5
local.identifier.doi10.1038/srep13492
local.identifier.scopusID2-s2.0-84940845248
local.type.statusPublished Version

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