ANU Open Research Repository has been upgraded. We are still working on a few minor issues, which may result in short outages throughout the day. Please get in touch with repository.admin@anu.edu.au if you experience any issues.
 

PEA-CLARITY: 3D molecular imaging of whole plant organs

Date

2015

Authors

Palmer, William M
Martin, Antony P
Flynn, Jamie R
Reed, Stephanie L.
White, Rosemary Gillian
Furbank, Robert
Grof, C.P.L.

Journal Title

Journal ISSN

Volume Title

Publisher

Nature Publishing Group

Abstract

Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating protein localisation of intact tissue in 3D whilst retaining cellular structure.

Description

Keywords

Citation

Source

Scientific Reports

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

DOI

10.1038/srep13492

Restricted until