Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains

Abstract

The VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemi