Biomaterials for spinal cord regeneration: outgrowth of presumptive neuronal precursors on electrospun poly(epsilon)-caprolactone scaffolds microlayered with alternating polyelectrolytes.
Date
2008
Authors
Thouas, George A.
Contreras, Karla G.
Bernard, Claude C
Sun, Gui Zhi
Tsang, Kelly M
Zhou, Kun
Nisbet, David
Forsythe, John S.
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Institute of Electrical and Electronics Engineers (IEEE Inc)
Abstract
The aim of this study was to assess the feasibility of electrospun poly(epsilon)-caprolactone (PCL) scaffolds treated with alternating paly-electrolytes as a controllable three-dimensional adhesive substrate for neuronal progenitors. Unmodified PCL surfaces were generally not supportive of mouse embryonic stem cell (mESC) colony adhesion. However, scaffolds surfaced using layer-by-layer (LbL) deposition of heparin/poly-L-lysine encouraged better local adhesion of mESC colonies, and networking of monolayers containing nestin-positive presumptive neurons, similar to laminin coated controls, as observed by immuno-fluorescence microscopy. Confocal microscopy further revealed depth-wise penetration of mESC nestin-positive cell populations, and orientation along grass topographical features in the LbL scaffolds. LbL deposition therefore appears to provide a satisfactory adhesive substrate for contact and mechanical guidance of neuronal outgrowth in three-dimensions.
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Keywords: biomaterial; electrolyte; heparin; polycaprolactone; polyester; polylysine; tissue scaffold; animal; article; cell adhesion; cell differentiation; cytology; embryonic stem cell; fluorescence microscopy; in vitro study; materials testing; mouse; nerve cell
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IEEE Engineering in Medicine and Biology Society: Conference Proceedings
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Journal article
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2037-12-31
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