Biomaterials for spinal cord regeneration: outgrowth of presumptive neuronal precursors on electrospun poly(epsilon)-caprolactone scaffolds microlayered with alternating polyelectrolytes.

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dc.contributor.authorThouas, George A.
dc.contributor.authorContreras, Karla G.
dc.contributor.authorBernard, Claude C
dc.contributor.authorSun, Gui Zhi
dc.contributor.authorTsang, Kelly M
dc.contributor.authorZhou, Kun
dc.contributor.authorNisbet, David
dc.contributor.authorForsythe, John S.
dc.date.accessioned2015-12-13T22:55:25Z
dc.date.issued2008
dc.date.updated2016-02-24T08:37:12Z
dc.description.abstractThe aim of this study was to assess the feasibility of electrospun poly(epsilon)-caprolactone (PCL) scaffolds treated with alternating paly-electrolytes as a controllable three-dimensional adhesive substrate for neuronal progenitors. Unmodified PCL surfaces were generally not supportive of mouse embryonic stem cell (mESC) colony adhesion. However, scaffolds surfaced using layer-by-layer (LbL) deposition of heparin/poly-L-lysine encouraged better local adhesion of mESC colonies, and networking of monolayers containing nestin-positive presumptive neurons, similar to laminin coated controls, as observed by immuno-fluorescence microscopy. Confocal microscopy further revealed depth-wise penetration of mESC nestin-positive cell populations, and orientation along grass topographical features in the LbL scaffolds. LbL deposition therefore appears to provide a satisfactory adhesive substrate for contact and mechanical guidance of neuronal outgrowth in three-dimensions.
dc.identifier.issn1557-170X
dc.identifier.urihttp://hdl.handle.net/1885/82523
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE Inc)
dc.sourceIEEE Engineering in Medicine and Biology Society: Conference Proceedings
dc.subjectKeywords: biomaterial; electrolyte; heparin; polycaprolactone; polyester; polylysine; tissue scaffold; animal; article; cell adhesion; cell differentiation; cytology; embryonic stem cell; fluorescence microscopy; in vitro study; materials testing; mouse; nerve cell
dc.titleBiomaterials for spinal cord regeneration: outgrowth of presumptive neuronal precursors on electrospun poly(epsilon)-caprolactone scaffolds microlayered with alternating polyelectrolytes.
dc.typeJournal article
local.bibliographicCitation.lastpage1828
local.bibliographicCitation.startpage1825
local.contributor.affiliationThouas, George A., Monash University
local.contributor.affiliationContreras, Karla G., Monash University
local.contributor.affiliationBernard, Claude C, Monash University
local.contributor.affiliationSun, Gui Zhi, Monash University
local.contributor.affiliationTsang, Kelly M, Monash University
local.contributor.affiliationZhou, Kun, Monash University
local.contributor.affiliationNisbet, David, College of Engineering and Computer Science, ANU
local.contributor.affiliationForsythe, John S., Monash University
local.contributor.authoremailu5031428@anu.edu.au
local.contributor.authoruidNisbet, David, u5031428
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor091299 - Materials Engineering not elsewhere classified
local.identifier.absseo970109 - Expanding Knowledge in Engineering
local.identifier.ariespublicationf5625xPUB10783
local.identifier.citationvolume2008
local.identifier.scopusID2-s2.0-65549166069
local.identifier.uidSubmittedByf5625
local.type.statusPublished Version

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