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In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings

Reichel, Marlene; Liao, Yalin; Rettel, Mandy; Ragan, Chikako; Evers, Maurits; Alleaume, Anne-Marie; Horos, Rastislav; Hentze, Matthias; Preiss, Thomas; Millar, Anthony

Description

RNA binding proteins (RBPs) control the fate and expression of a transcriptome. Despite this fundamental importance, our understanding of plant RBPs is rudimentary, being mainly derived via bioinformatic extrapolation from other kingdoms. Here, we adapted the mRNA-protein interactome capture method to investigate the RNA binding proteome in planta. From Arabidopsis thaliana etiolated seedlings, we captured more than 700 proteins, including 300 with high confidence that we have defined as the...[Show more]

CollectionsANU Research Publications
Date published: 2016
Type: Journal article
URI: http://hdl.handle.net/1885/267297
Source: The Plant Cell
DOI: 10.1105/tpc.16.00562

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