In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings

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dc.contributor.authorReichel, Marlene
dc.contributor.authorLiao, Yalin
dc.contributor.authorRettel, Mandy
dc.contributor.authorRagan, Chikako
dc.contributor.authorEvers, Maurits
dc.contributor.authorAlleaume, Anne-Marie
dc.contributor.authorHoros, Rastislav
dc.contributor.authorHentze, Matthias
dc.contributor.authorPreiss, Thomas
dc.contributor.authorMillar, Anthony
dc.date.accessioned2022-06-15T04:29:09Z
dc.date.issued2016
dc.date.updated2021-02-14T07:21:23Z
dc.description.abstractRNA binding proteins (RBPs) control the fate and expression of a transcriptome. Despite this fundamental importance, our understanding of plant RBPs is rudimentary, being mainly derived via bioinformatic extrapolation from other kingdoms. Here, we adapted the mRNA-protein interactome capture method to investigate the RNA binding proteome in planta. From Arabidopsis thaliana etiolated seedlings, we captured more than 700 proteins, including 300 with high confidence that we have defined as the At-RBP set. Approximately 75% of these At-RBPs are bioinformatically linked with RNA biology, containing a diversity of canonical RNA binding domains (RBDs). As no prior experimental RNA binding evidence exists for the majority of these proteins, their capture now authenticates them as RBPs. Moreover, we identified protein families harboring emerging and potentially novel RBDs, including WHIRLY, LIM, ALBA, DUF1296, and YTH domain-containing proteins, the latter being homologous to animal RNA methylation readers. Other At-RBP set proteins include major signaling proteins, cytoskeleton-associated proteins, membrane transporters, and enzymes, suggesting the scope and function of RNA-protein interactions within a plant cell is much broader than previously appreciated. Therefore, our foundation data set has provided an unbiased insight into the RNA binding proteome of plants, on which future investigations into plant RBPs can be based.en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn1040-4651en_AU
dc.identifier.urihttp://hdl.handle.net/1885/267297
dc.language.isoen_AUen_AU
dc.publisherAmerican Society of Plant Biologistsen_AU
dc.relationhttp://purl.org/au-research/grants/nhmrc/1045417en_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP110103493en_AU
dc.rights© 2016 The authorsen_AU
dc.sourceThe Plant Cellen_AU
dc.titleIn Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlingsen_AU
dc.typeJournal articleen_AU
local.bibliographicCitation.issue10en_AU
local.bibliographicCitation.lastpage2452en_AU
local.bibliographicCitation.startpage2435en_AU
local.contributor.affiliationReichel, Marlene, College of Science, ANUen_AU
local.contributor.affiliationLiao, Yalin, College of Health and Medicine, ANUen_AU
local.contributor.affiliationRettel, Mandy, European Molecular Biology Laboratoryen_AU
local.contributor.affiliationRagan, Chikako, College of Health and Medicine, ANUen_AU
local.contributor.affiliationEvers, Maurits, College of Health and Medicine, ANUen_AU
local.contributor.affiliationAlleaume, Anne-Marie, European Molecular Biology Laboratoryen_AU
local.contributor.affiliationHoros, Rastislav, European Molecular Biology Laboratoryen_AU
local.contributor.affiliationHentze, Matthias, EMBL, Germanyen_AU
local.contributor.affiliationPreiss, Thomas, College of Health and Medicine, ANUen_AU
local.contributor.affiliationMillar, Anthony, College of Science, ANUen_AU
local.contributor.authoremailu5114881@anu.edu.auen_AU
local.contributor.authoruidReichel, Marlene, u4791315en_AU
local.contributor.authoruidLiao, Yalin, u5114881en_AU
local.contributor.authoruidRagan, Chikako, u1002094en_AU
local.contributor.authoruidEvers, Maurits, u2528469en_AU
local.contributor.authoruidPreiss, Thomas, u5046545en_AU
local.contributor.authoruidMillar, Anthony, u4325116en_AU
local.description.embargo2099-12-31
local.description.notesImported from ARIESen_AU
local.identifier.absfor060405 - Gene Expression (incl. Microarray and other genome-wide approaches)en_AU
local.identifier.absfor060102 - Bioinformaticsen_AU
local.identifier.absfor060702 - Plant Cell and Molecular Biologyen_AU
local.identifier.ariespublicationu3355791xPUB4en_AU
local.identifier.citationvolume28en_AU
local.identifier.doi10.1105/tpc.16.00562en_AU
local.identifier.scopusID2-s2.0-84995486911
local.identifier.thomsonID000390135400011
local.identifier.uidSubmittedByu3355791en_AU
local.publisher.urlhttps://academic.oup.com/en_AU
local.type.statusPublished Versionen_AU

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