Backbone assignment of fully protonated solid proteins by 1 H detection and ultrafast magic-angle-spinning NMR spectroscopy
dc.contributor.author | Marchetti, Alessandro | |
dc.contributor.author | Jehle, Stefan | |
dc.contributor.author | Felletti, Michele | |
dc.contributor.author | Knight, Michael J. | |
dc.contributor.author | Wang, Yao | |
dc.contributor.author | Xu, Zhi-Qiang | |
dc.contributor.author | Park, Ah Young | |
dc.contributor.author | Lesage, Anne | |
dc.contributor.author | Emsley, Lyndon | |
dc.contributor.author | Dixon, Nicholas Edward | |
dc.contributor.author | Pintacuda, Guido | |
dc.contributor.author | Otting, Gottfried | |
dc.date.accessioned | 2015-12-07T22:30:42Z | |
dc.date.issued | 2012 | |
dc.date.updated | 2016-02-24T11:39:27Z | |
dc.description.abstract | Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities. | |
dc.identifier.issn | 1433-7851 | |
dc.identifier.uri | http://hdl.handle.net/1885/22428 | |
dc.publisher | Wiley-VCH Verlag GMBH | |
dc.source | Angewandte Chemie International Edition | |
dc.subject | Keywords: Deuterations; DNA polymerase; Magic-angle-spinning NMR; Noncrystalline proteins; Protein samples; Protein structures; Protonated; scalar transfers; Solid protein; Ultra-fast; Deuterium; Nuclear magnetic resonance spectroscopy; Proteins; Protonation; Magic DNA polymerase; magic-angle spinning; NMR spectroscopy; protein structures; scalar transfers | |
dc.title | Backbone assignment of fully protonated solid proteins by 1 H detection and ultrafast magic-angle-spinning NMR spectroscopy | |
dc.type | Journal article | |
local.bibliographicCitation.issue | 43 | |
local.bibliographicCitation.lastpage | 10759 | |
local.bibliographicCitation.startpage | 10756 | |
local.contributor.affiliation | Marchetti, Alessandro, University of Lyon | |
local.contributor.affiliation | Jehle, Stefan, University of Lyon | |
local.contributor.affiliation | Felletti, Michele, University of Lyon | |
local.contributor.affiliation | Knight, Michael J., University of Lyon | |
local.contributor.affiliation | Wang, Yao, University of Wollongong | |
local.contributor.affiliation | Xu, Zhi-Qiang, University of Wollongong | |
local.contributor.affiliation | Park, Ah Young, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Otting, Gottfried, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Lesage, Anne, University of Lyon | |
local.contributor.affiliation | Emsley, Lyndon, University of Lyon | |
local.contributor.affiliation | Dixon, Nicholas Edward, University of Wollongong | |
local.contributor.affiliation | Pintacuda, Guido, Ecole Normale Superieure de Lyon | |
local.contributor.authoremail | u4046684@anu.edu.au | |
local.contributor.authoruid | Park, Ah Young, u4015196 | |
local.contributor.authoruid | Otting, Gottfried, u4046684 | |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | |
local.identifier.absfor | 030403 - Characterisation of Biological Macromolecules | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
local.identifier.ariespublication | u8302325xPUB21 | |
local.identifier.ariespublication | U4217927xPUB818 | |
local.identifier.citationvolume | 51 | |
local.identifier.doi | 10.1002/anie.201203124 | |
local.identifier.scopusID | 2-s2.0-84867548984 | |
local.identifier.thomsonID | 000310076100012 | |
local.identifier.uidSubmittedBy | u8302325 | |
local.type.status | Published Version |
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