Arbitrary multi-site two-photon excitation in four dimensions
Date
2009-09-02
Authors
Daria, Vincent Ricardo
Stricker, Christian
Bowman, Richard
Redman, Stephen
Bachor, Hans
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Volume Title
Publisher
American Institute of Physics (AIP)
Abstract
We demonstrate dynamic and arbitrary multisite two-photon excitation in three
dimensions using the holographic projection method. Rapid response (fourth
dimension) is achieved through high-speed noniterative calculation of the
hologram using a video graphics accelerator board. We verify that the projected
asymmetric spot configurations have sufficient spatiotemporal photon density
for localized two-photon excitation. This system is a significant advance and
can be applied to time-resolved photolysis of caged compounds in biological
cells and complex neuronal networks, nonlinear microfabrication and volume
holographic optical storage.
Description
Keywords
Keywords: Biological cells; Graphics accelerators; High-speed; Holographic projection; Multi-site; Neuronal networks; Non-iterative; Optical Storage; Photon densities; Rapid response; Three dimensions; Time-resolved; Two-photon excitations; Volume holographic; Phot
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Source
Applied Physics Letters
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Journal article
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