Arbitrary multi-site two-photon excitation in four dimensions

Date

2009-09-02

Authors

Daria, Vincent Ricardo
Stricker, Christian
Bowman, Richard
Redman, Stephen
Bachor, Hans

Journal Title

Journal ISSN

Volume Title

Publisher

American Institute of Physics (AIP)

Abstract

We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

Description

Keywords

Keywords: Biological cells; Graphics accelerators; High-speed; Holographic projection; Multi-site; Neuronal networks; Non-iterative; Optical Storage; Photon densities; Rapid response; Three dimensions; Time-resolved; Two-photon excitations; Volume holographic; Phot

Citation

Source

Applied Physics Letters

Type

Journal article

Book Title

Entity type

Access Statement

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DOI

10.1063/1.3216581

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