A family of RS domain proteins with novel subcellular localization and trafficking

Date

2005-03-01

Authors

Kavanagh, Steven J
Schulz, Thomas C
Davey, Philippa
Claudianos, Charles
Russell, Carrie
Rathjen, Peter D

Journal Title

Journal ISSN

Volume Title

Publisher

Oxford University Press

Abstract

We report the sequence, conservation and cell biology of a novel protein, Psc1, which is expressed and regulated within the embryonic pluripotent cell population of the mouse. The Psc1 sequence includes an RS domain and an RNA recognition motif (RRM), and a sequential arrangement of protein motifs that has not been demonstrated for other RS domain proteins. This arrangement was conserved in a second mouse protein (BAC34721). The identification of Psc1 and BAC34721 homologues in vertebrates and related proteins, more widely throughout evolution, defines a new family of RS domain proteins termed acidic rich RS (ARRS) domain proteins. Psc1 incorporated into the nuclear speckles, but demonstrated novel aspects of subcellular distribution including localization to speckles proximal to the nuclear periphery and localization to punctate structures in the cytoplasm termed cytospeckles. Integration of Psc1 into cytospeckles was dependent on the RRM. Cytospeckles were dynamic within the cytoplasm and appeared to traffic into the nucleus. These observations suggest a novel role in RNA metabolism for ARRS proteins.

Description

Keywords

Keywords: gene product; protein Psc1; unclassified drug; complementary DNA; nuclear protein; protein; Psc1 protein, mouse; RNA binding protein; amino acid sequence; animal cell; article; bacterial artificial chromosome; cell nucleus; cell organelle; cell population

Citation

Nucleic Acids Research 33.4 (2005): 1309-1322

Source

Nucleic Acids Research

Type

Journal article

Book Title

Entity type

Access Statement

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DOI

10.1093/nar/gki269

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