Calcium spikes in basal dendrites of layer 5 pyramidal neurons during action potential bursts

Date

2006

Authors

Kampa, Bjoern
Stuart, Gregory J

Journal Title

Journal ISSN

Volume Title

Publisher

Society for Neuroscience

Abstract

Patch-clamp recording from dendrites has lead to a significant increase in our understanding of the mechanisms underlying signal integration and propagation in neurons. The majority of synaptic input to neurons, however, is made onto small-diameter dendrites, currently beyond the scope of patch-clamp recording techniques. Here we use both calcium and voltage imaging to investigate propagation of action potentials (APs) in fine basal dendrites of cortical layer 5 pyramidal neurons. High-frequency (200 Hz) AP bursts caused supralinear increases in dendritic calcium at distal, but not proximal, basal locations. Supralinear increases in dendritic calcium were also observed at distal basal locations during AP trains above a critical frequency (∼100 Hz). Using voltage imaging, we show that single APs undergo significant attenuation as they propagate into basal dendrites, whereas AP bursts lead to generation of dendritic calcium spikes. Focal and bath application of 4-AP increased the amplitude of calcium transients evoked by APs at distal, but not proximal, locations, suggesting that A-type potassium channels regulate AP backpropagation into basal dendrites. Finally, we show that pairing EPSPs with AP bursts is an effective means of activating synaptic NMDA receptors in basal dendrites. The experimental observations on the role of A-type potassium channels in regulation of AP backpropagation in basal dendrites, as well as the generation of dendritic calcium spikes during AP bursts, were reproduced in a morphologically realistic neuronal model with uniform distributions of dendritic sodium, calcium, and potassium channels. Together, these findings have important implications for understanding dendritic integration and synaptic plasticity in cortical basal dendrites.

Description

Keywords

Keywords: calcium; n methyl dextro aspartic acid receptor; potassium channel; sodium channel; voltage gated calcium channel; action potential; animal cell; animal experiment; animal tissue; article; controlled study; dendrite; mouse; nerve cell plasticity; nerve ex Action potential; Backpropagation; Dendrite; Imaging; Neocortex; STDP

Citation

Source

Journal of Neuroscience

Type

Journal article

Book Title

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DOI

10.1523/JNEUROSCI.3062-05.2006

Restricted until

2037-12-31