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Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici

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dc.contributor.authorMcDonald, Megan
dc.contributor.authorWilliams , Angela H
dc.contributor.authorMilgate, Andrew
dc.contributor.authorPattemore, Julie A.
dc.contributor.authorSolomon, Peter
dc.contributor.authorHane , James K
dc.date.accessioned2015-12-13T22:36:22Z
dc.date.issued2015
dc.date.updated2015-12-11T09:31:26Z
dc.description.abstractThe wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessory chromosomes. The IPO323 reference, when combined with low-cost next-generation sequencing and bioinformatics, can be used as a powerful tool to assess the presence or absence of accessory chromosomes. We present an outline of a range of bioinformatics techniques that can be applied to the analysis of presence-absence variation among accessory chromosomes across 13 novel isolates of Z. tritici.
dc.identifier.issn1087-1845
dc.identifier.urihttp://hdl.handle.net/1885/76723
dc.publisherAcademic Press
dc.sourceFungal Genetics and Biology
dc.titleNext-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici
dc.typeJournal article
local.bibliographicCitation.lastpage75
local.bibliographicCitation.startpage71
local.contributor.affiliationMcDonald, Megan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWilliams , Angela H , Curtin University
local.contributor.affiliationMilgate, Andrew, NSW Department of Primary Industries
local.contributor.affiliationPattemore, Julie A., Charles Sturt University
local.contributor.affiliationSolomon, Peter, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHane , James K , Curtin University
local.contributor.authoruidMcDonald, Megan, u5261870
local.contributor.authoruidSolomon, Peter, u4632004
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060102 - Bioinformatics
local.identifier.absfor060704 - Plant Pathology
local.identifier.absfor060505 - Mycology
local.identifier.absseo820507 - Wheat
local.identifier.ariespublicationU3488905xPUB5532
local.identifier.citationvolume79
local.identifier.doi10.1016/j.fgb.2015.04.012
local.identifier.scopusID2-s2.0-84937564837
local.type.statusPublished Version

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