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A re-investigation of the path of carbon in photosynthesis utilizing GC/MS methodology. Unequivocal verification of the participation of octulose phosphates in the pathway

Date

Authors

Flanigan, Ian

MacLeod, John

Williams, John F

Publisher

Kluwer Academic Publishers

Abstract

A GC/EIMS/SIM methodology has been developed to re-examine the path of carbon in photosynthesis. Exposing isolated spinach chloroplasts to13CO2 on a solid support for a defined period followed by quenching and work-up provided a mixture of labelled sugar

Keywords

Keywords: carbon; D glycero D altro octulose 1,8 bisphosphate; D-glycero-D-altro-octulose 1,8-bisphosphate; D-glycero-D-ido-octulose 1,8-bisphosphate; ketose; octulose 1,8 diphosphate; oxygen; sugar phosphate; unclassified drug; article; calibration; chloroplast; m 13C-enriched sugar phosphates; 13CO 2 incorporation; Chloroplasts; Gas chromatography-mass spectrometry; Modified Calvin cycle; Mox-TMS derivatives; Octulose phosphate shunt; Selected ion monitoring

URI

http://hdl.handle.net/1885/22505

Collections

ANU Research Publications

Source

Photosynthesis Research

Type

Journal article

DOI

10.1007/s11120-006-9114-4

Restricted until

2037-12-31

Downloads

File

Description

01_Flanigan_A_re-investigation_of_the_path_2006.pdf (282.69 KB)