Evidence against the existence of genes for resistance to Fusarium oxysporum f. sp. lycopersici races 1 and 2 on Solanum pennellii chromosome 7 additional to I-3

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dc.contributor.authorDo, Huong
dc.contributor.authorCatanzariti, Ann-Maree
dc.contributor.authorLim, Ginny
dc.contributor.authorJones, David
dc.date.accessioned2019-04-21T03:56:41Z
dc.date.issued2018
dc.date.updated2019-03-12T07:32:31Z
dc.description.abstractThe I-3 gene for resistance to Fusarium oxysporum f. sp. lycopersici races 2 and 3 was introgressed into tomato (Solanum lycopersicum) from its wild relative Solanum pennellii. Additional genes for resistance to races 1 and 2 are suggested to exist alongside I-3 on S. pennellii chromosome 7. To examine the existence of a separate gene for race 2 resistance, we tested recombinants generated during the isolation of I-3 that lacked I-3 but carried the region suggested to confer race 2 resistance. Disease assays showed these lines had no resistance to race 2. To confirm this result, we tested the introgression lines IL7-2 and IL7-4, which together cover the entirety of S. pennellii chromosome 7 (including I-3), for resistance to race 2 carrying a knockout of Avr3. Loss of Avr3 resulted in a loss of race 2 resistance, indicating that I-3 is the only gene conferring race 2 resistance in these two lines. To examine the existence of a gene for race 1 resistance (designated I-1 in the literature), we tested the tomato line E779, which carries I-3 but lacks the I gene conferring race 1 resistance. Disease assays showed E779 had intermediate resistance to race 1, but no resistance to the Avr3-knockout derivative, indicating that I-3 alone is able to confer race 1 resistance. Finally, we examined the race-1-resistant tomato line 90E402F, which is supposed to carry I-1, for the presence of S. pennellii markers on chromosome 7. We found no S. pennellii markers in the region supposed to carry I-1, but instead found that this line carries the I gene. Thus, we found no evidence to support the existence of additional genes for race 1 and 2 resistance on S. pennellii chromosome 7, but instead found that I-3 alone is sufficient to explain the observed resistance to our isolates of races 1, 2 and 3.en_AU
dc.description.sponsorshipHTTD was supported by an Australia Awards Scholarship. A.-M.C. was funded by an Australian Research Council postdoctoral fellowship (DP1095157).
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn0567-7572en_AU
dc.identifier.urihttp://hdl.handle.net/1885/160526
dc.language.isoen_AUen_AU
dc.publisherInternational Society for Horticultural Scienceen_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP1095157
dc.sourceActa Horticulturaeen_AU
dc.titleEvidence against the existence of genes for resistance to Fusarium oxysporum f. sp. lycopersici races 1 and 2 on Solanum pennellii chromosome 7 additional to I-3en_AU
dc.typeJournal articleen_AU
local.contributor.affiliationDo, Huong, College of Science, ANUen_AU
local.contributor.affiliationCatanzariti, Ann-Maree, College of Science, ANUen_AU
local.contributor.affiliationLim, Ginny, College of Science, ANUen_AU
local.contributor.affiliationJones, David, College of Science, ANUen_AU
local.contributor.authoremailu4017719@anu.edu.auen_AU
local.contributor.authoruidDo, Huong, u4884767en_AU
local.contributor.authoruidCatanzariti, Ann-Maree, u4017719en_AU
local.contributor.authoruidLim, Ginny, u4057877en_AU
local.contributor.authoruidJones, David, u9614975en_AU
local.description.embargo2040-01-01
local.description.notesImported from ARIESen_AU
local.identifier.absfor060704 - Plant Pathologyen_AU
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciencesen_AU
local.identifier.ariespublicationu4485658xPUB206en_AU
local.identifier.citationvolume1207en_AU
local.identifier.doi10.17660/ActaHortic.2018.1207.2en_AU
local.identifier.scopusID2-s2.0-85052569325
local.identifier.uidSubmittedByu4485658en_AU
local.type.statusPublished Versionen_AU

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