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Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system

Date

Authors

Cowieson, Nathan

Listwan, Pawel

Kurz, Mareike

Aagaard, Anna

Ravasi, Timothy

Wells, Christine

Hume, D A

Kobe, Bostjan

Martin, Jennifer Louise

Huber, Thomas

Publisher

Springer

Abstract

We investigated the parallel production in medium throughput of mouse proteins, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. The methods were scaled up to allow the simultaneous processing of

Keywords

Keywords: recombinant protein; article; automation; bacterium; chemical modification; eukaryote; gene expression system; gene targeting; molecular cloning; nonhuman; priority journal; protein expression; protein targeting; solubility; Animals; Cloning, Molecular; E Bacterial expression; Mammalian proteins; Medium-throughput; Protein expression screening; Recombinatorial cloning

URI

http://hdl.handle.net/1885/61581

Collections

ANU Research Publications

Source

Journal of Structural and Functional Genomics

Type

Journal article

DOI

10.1007/s10969-005-0462-7

Restricted until

2037-12-31

Downloads

File

Description

01_Cowieson_Pilot_studies_on_the_parallel_2005.pdf (225.6 KB)

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