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In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy

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dc.contributor.authorHirst, William
dc.contributor.authorKiefer, Christine
dc.contributor.authorAbdosamadi, Mohammad Kazem
dc.contributor.authorSchäffer, Erik
dc.contributor.authorReber, Simone
dc.date.accessioned2022-11-08T00:41:47Z
dc.date.available2022-11-08T00:41:47Z
dc.date.issued2020-12-18
dc.date.updated2021-11-28T07:26:44Z
dc.description.abstractDynamic microtubules are essential for many processes in the lives of eukaryotic cells. To study and understand the mechanisms of microtubule dynamics and regulation, in vitro reconstitution with purified components has proven a vital approach. Imaging microtubule dynamics can be instructive for a given species, isoform composition, or biochemical modification. Here, we describe two methods that visualize microtubule dynamics at high speed and high contrast: (1) total internal reflection fluorescence microscopy and (2) label-free interference reflection microscopy. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).en_AU
dc.description.sponsorshipS.R. acknowledges funding by the IRI Life Sciences (Humboldt-Universitat zu Berlin, Excellence Initiative/DFG). W.H. was supported by the Alliance Berlin Canberra co-funded by a grant from the Deutsche Forschungsgemeinschaft (DFG) for the International Research Training Group (IRTG) 2290 and the Australian National University. C.K. thanks the Deutsche Forschungsgesellschaft (DFG, JA 2589/1-1).en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn2666-1667en_AU
dc.identifier.urihttp://hdl.handle.net/1885/278333
dc.language.isoen_AUen_AU
dc.provenanceThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_AU
dc.publisherCell Pressen_AU
dc.rights© 2020 The Authorsen_AU
dc.rights.licenseCreative Commons BY-NC-ND licenseen_AU
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_AU
dc.sourceSTAR Protocolsen_AU
dc.titleIn Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopyen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue3en_AU
local.bibliographicCitation.lastpage26en_AU
local.bibliographicCitation.startpage1en_AU
local.contributor.affiliationHirst, William, College of Science, ANUen_AU
local.contributor.affiliationKiefer, Christine, Universität Tübingenen_AU
local.contributor.affiliationAbdosamadi, Mohammad Kazem, Universität Tübingenen_AU
local.contributor.affiliationSchäffer, Erik, Universität Tübingenen_AU
local.contributor.affiliationReber, Simone, Humboldt University of Berlinen_AU
local.contributor.authoruidHirst, William, u6828887en_AU
local.description.notesImported from ARIESen_AU
local.identifier.absfor320704 - Medical parasitologyen_AU
local.identifier.absfor310702 - Infectious agentsen_AU
local.identifier.absfor321401 - Basic pharmacologyen_AU
local.identifier.ariespublicationa383154xPUB20639en_AU
local.identifier.citationvolume1en_AU
local.identifier.doi10.1016/j.xpro.2020.100177en_AU
local.identifier.scopusID2-s2.0-85108503090
local.publisher.urlhttps://www.sciencedirect.com/en_AU
local.type.statusPublished Versionen_AU

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