Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring

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dc.contributor.authorGregory, Stephen L
dc.contributor.authorEbrahimi, Saman
dc.contributor.authorMilverton, Joanne
dc.contributor.authorJones, Whitney M
dc.contributor.authorBejsovec, Amy
dc.contributor.authorSaint, Robert
dc.date.accessioned2015-12-10T22:58:24Z
dc.date.issued2008
dc.date.updated2015-12-10T08:06:19Z
dc.description.abstractThe mitotic microtubule array plays two primary roles in cell division. It acts as a scaffold for the congression and separation of chromosomes, and it specifies and maintains the contractile-ring position. The current model for initiation of Drosophila and mammalian cytokinesis [1-5] postulates that equatorial localization of a RhoGEF (Pbl/Ect2) by a microtubule-associated motor protein complex creates a band of activated RhoA [6], which subsequently recruits contractile-ring components such as actin, myosin, and Anillin [1-3]. Equatorial microtubules are essential for continued constriction, but how they interact with the contractile apparatus is unknown. Here, we report the first direct molecular link between the microtubule spindle and the actomyosin contractile ring. We find that the spindle-associated component, RacGAP50C, which specifies the site of cleavage [1-5], interacts directly with Anillin, an actin and myosin binding protein found in the contractile ring [7-10]. Both proteins depend on this interaction for their localization. In the absence of Anillin, the spindle-associated RacGAP loses its association with the equatorial cortex, and cytokinesis fails. These results account for the long-observed dependence of cytokinesis on the continual presence of microtubules at the cortex.
dc.identifier.issn0960-9822
dc.identifier.urihttp://hdl.handle.net/1885/60840
dc.publisherCell Press
dc.sourceCurrent Biology
dc.subjectKeywords: anillin; contractile protein; Drosophila protein; guanosine triphosphatase activating protein; myosin adenosine triphosphatase; RacGap50C protein, Drosophila; unclassified drug; animal; article; cell division; cytology; Drosophila; fluorescence resonance CELLCYCLE
dc.titleCell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring
dc.typeJournal article
local.bibliographicCitation.lastpage29
local.bibliographicCitation.startpage25
local.contributor.affiliationGregory, Stephen L, University of Adelaide
local.contributor.affiliationEbrahimi, Saman, University of Adelaide
local.contributor.affiliationMilverton, Joanne, University of Adelaide
local.contributor.affiliationJones, Whitney M, Duke University
local.contributor.affiliationBejsovec, Amy, Duke University
local.contributor.affiliationSaint, Robert, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidSaint, Robert, u4042812
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060402 - Cell and Nuclear Division
local.identifier.absfor060103 - Cell Development, Proliferation and Death
local.identifier.ariespublicationu9204316xPUB567
local.identifier.citationvolume18
local.identifier.doi10.1016/j.cub.2007.11.050
local.identifier.scopusID2-s2.0-38749114059
local.identifier.uidSubmittedByu9204316
local.type.statusPublished Version

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