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A single-step assay for the Gerbich-negative allele of glycophorin C

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dc.contributor.authorScott, Brendan
dc.contributor.authorEasteal, Simon
dc.date.accessioned2015-12-08T22:26:50Z
dc.date.issued2008
dc.date.updated2015-12-08T09:14:54Z
dc.description.abstractThe Gerbich erythrocyte surface protein, glycophorin C (GYPC), can be used by Plasmodium falciparum to invade erythrocytes. The Melanesian Gerbich-negative antigenic condition (Ge(-)) is frequent in some populations where malaria is endemic, suggesting that it protects against malaria. We have determined as precisely as possible the breakpoint of the chromosomal deletion that causes the Ge(-) condition by comparing the partial GYPC sequence of a Papuan Ge(-/-) homozygous individual with known sequences of GYPC. This localisation has allowed us to develop a robust single-step PCR assay suitable for rapid screening of Ge(-). This method is easier to implement than existing methods, can reliably identify heterozygous individuals, and will considerably aid efforts to study the distribution of Ge(-) and its role in protection against malaria.
dc.identifier.issn1079-9796
dc.identifier.urihttp://hdl.handle.net/1885/33801
dc.publisherAcademic Press
dc.sourceBlood cells, Molecules and Diseases
dc.subjectKeywords: glycophorin C; allele; article; chromosome deletion; erythrocyte; gene sequence; genotype; human; malaria; nucleotide sequence; Plasmodium falciparum; priority journal; Alleles; Animals; Base Sequence; Erythrocyte Membrane; Exons; Genetic Predisposition t Gerbich; Glycophorin; Malaria; Papua; Sialic
dc.titleA single-step assay for the Gerbich-negative allele of glycophorin C
dc.typeJournal article
local.bibliographicCitation.issue1
local.bibliographicCitation.lastpage4
local.bibliographicCitation.startpage1
local.contributor.affiliationScott, Brendan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationEasteal, Simon, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidScott, Brendan, a241381
local.contributor.authoruidEasteal, Simon, u8200596
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor110309 - Infectious Diseases
local.identifier.ariespublicationu4020362xPUB106
local.identifier.citationvolume41
local.identifier.doi10.1016/j.bcmd.2008.02.009
local.identifier.scopusID2-s2.0-44449132501
local.identifier.thomsonID000256785600001
local.type.statusPublished Version

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