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XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

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Date

2016

Authors

Thomas, Nicholas
Schwessinger, Benjamin
Liu, Furong
Chen , Huamin
wei, Tong
Nguyen, Yen P.
Shaker, Isaac W.F.
Ronald , Pamela

Journal Title

Journal ISSN

Volume Title

Publisher

PeerJ

Abstract

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

Description

Keywords

Citation

URI

http://hdl.handle.net/1885/153785

Collections

ANU Research Publications

Source

PeerJ

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

DOI

10.7717/peerj.2446

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