The French press: a repeatable and high-throughput roach to exercising zebrafish (Danio rerio)

Date

2018-01-17

Authors

Usui, Takuji
Noble, Daniel W. A.
O'Dea, Rose E.
Fangmeier, Melissa L.
Lagisz, M.
Hesselson, Daniel
Nakagawa, Shinichi

Journal Title

Journal ISSN

Volume Title

Publisher

PeerJ

Abstract

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.

Description

Keywords

Burst swimming, Endurance test, Sustained swimming, Technique, Circular

Citation

Usui T, Noble DWA, O’Dea RE, Fangmeier ML, Lagisz M, Hesselson D, Nakagawa S. 2018. The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio) PeerJ 6:e4292 https://doi.org/10.7717/peerj.4292

Source

PeerJ

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

Creative Commons Attribution License

DOI

10.7717/peerj.4292

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