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Anticipating and Identifying Collateral Damage in Genome Editing

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dc.contributor.authorBurgio, Gaetan
dc.contributor.authorTeboul, Lydia
dc.date.accessioned2022-10-04T03:25:03Z
dc.date.available2022-10-04T03:25:03Z
dc.date.issued2020
dc.date.updated2021-11-28T07:21:02Z
dc.description.abstractGenome editing has a transformative potential in healthcare or to improve crops or livestock. However, the use of Cas9 or other nucleases can yield unpredictable events at the target site or off target. To overcome these challenge, it is critical to understand and accurately predict the whole range of possible editing outcomes. The key to success is to combine molecular assays to evaluate the sequence changes at the target site and to quantify the number of copies of segments deleted/inserted across the genome. For all applications, thorough evaluation of these outcomes is essential to identify all collateral damage from nuclease activity and for a real appraisal of the benefits and risks associated with applying this technology.en_AU
dc.description.sponsorshipL.T. was supported by Medical Research Council in the UK (grant A410) and the National Institute for Health in the USA (Grant U42OD011174). G.B. was supported by the National collaborative Research Infrastructure (NCRIS) via the Australian Phenomics Network, the National Health and Medical Research Council (NHMRC) of Australia (Grant APP1143008), the Australian Research Council (ARC) (Grant DP180101494), and the National Natural Science Foundation of China (NSFC) (Grant 81772214).en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn0168-9525en_AU
dc.identifier.urihttp://hdl.handle.net/1885/274269
dc.language.isoen_AUen_AU
dc.provenanceThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_AU
dc.publisherElsevieren_AU
dc.relationhttp://purl.org/au-research/grants/nhmrc/1143008en_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP180101494en_AU
dc.rights© 2020 Elsevier Ltd.en_AU
dc.rights.licenseCC BY-NC-ND licenseen_AU
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_AU
dc.sourceTrends in Geneticsen_AU
dc.titleAnticipating and Identifying Collateral Damage in Genome Editingen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue12en_AU
local.bibliographicCitation.lastpage914en_AU
local.bibliographicCitation.startpage905en_AU
local.contributor.affiliationBurgio, Gaetan, College of Health and Medicine, ANUen_AU
local.contributor.affiliationTeboul, Lydia , Medical Research Council Harwell Institute, Harwell Campusen_AU
local.contributor.authoruidBurgio, Gaetan, u5727247en_AU
local.description.notesImported from ARIESen_AU
local.identifier.absfor320603 - Medical molecular engineering of nucleic acids and proteinsen_AU
local.identifier.absfor320601 - Gene and molecular therapyen_AU
local.identifier.absfor310113 - Synthetic biologyen_AU
local.identifier.absseo240803 - Human pharmaceutical treatmentsen_AU
local.identifier.absseo280103 - Expanding knowledge in the biomedical and clinical sciencesen_AU
local.identifier.absseo200105 - Treatment of human diseases and conditionsen_AU
local.identifier.ariespublicationa383154xPUB14943en_AU
local.identifier.citationvolume36en_AU
local.identifier.doi10.1016/j.tig.2020.09.011en_AU
local.publisher.urlhttps://www.elsevier.com/en-auen_AU
local.type.statusPublished Versionen_AU

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