Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)
Date
2020
Authors
Orton, Henry
Stanek, Jan
Schubeis, Tobias
Foucaudeau, Dylan
Ollier, Claire
Draney, Adrian W
Le Marchand, Tanguy
Cala-De Paepe, Diane
Felli, Isabella C
Pierattelli, Roberta
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.
Description
Keywords
nmr spectroscopy, automation, projection spectroscopy, proton detection, solid-state structures, automation, isotope labeling, nuclear magnetic resonance, biomolecular, proteins, superoxide dismutase, beta 2-microglobulin
Citation
Collections
Source
Angewandte Chemie
Type
Journal article
Book Title
Entity type
Access Statement
Open Access