Role of decay-accelerating factor in regulating survival of human cervical cancer cells




Gao, Ling-Juan
Ding, Lan
Guo, Shu-Yu
Cai, You-Qun
Su, Ya-Juan
Gong, Hui
Liu, Yun
Chen, Chen
Gu, Ping-Qing

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Springer Verlag


Background: Decay-accelerating factor (DAF) is one of the key molecules involved in cell protection against autologous complement, which restricts the action of complement at critical stages of the cascade reaction. The effect of DAF on the survival of human cervical cancer cell (ME180) has not been demonstrated. Methods: In this study we applied, for the first time, small interference RNA (siRNA) to knock down the expression of the DAF with the aim of exploiting complement more effectively for tumor cell damage. Meanwhile, we investigated the effects of DAF on the viability and migration, moreover the proliferation of ME180 cell. Results: The results showed that the expression of DAF was significantly increased in human cervical cancer tissues. SiRNA inhibition of DAF expression enhanced complement-dependent cytolysis up to 32% in ME180 cells, which contributed to the control of C3 activation and increased the cells viability, migration and augment the number of ME180 cells. Conclusion: These data indicated that DAF siRNA described in this study may offer an additional alternative to improve the efficacy of antibody- and complement-based cancer immunotherapy.



Keywords: complement component C3; decay accelerating factor; small interfering RNA; article; cancer cell; cell migration; cell proliferation; cell survival; cell viability; controlled study; female; gene silencing; gene targeting; human; human cell; human tissue; Decay-accelerating factor (DAF); Human cervical cancer cell (ME180); Small interference RNA (siRNA)



Journal of Cancer Research and Clinical Oncology


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