Estimation of Photorespiratory Fluxes by Gas Exchange

Date

2017

Authors

Busch, Florian
Deans, Ross
Holloway-Phillips, Meisha-Marika

Journal Title

Journal ISSN

Volume Title

Publisher

Humana Press

Abstract

Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78–90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its 12CO2 and 13CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using 13CO2.

Description

Keywords

Photorespiration, Photosynthetic gas exchange, Membrane-inlet mass spectrometry, Carbon isotopes, Photosynthesis model, Rubisco kinetics

Citation

Source

Type

Book chapter

Book Title

Photorespiration Methods and Protocols

Entity type

Access Statement

License Rights

DOI

10.1007/978-1-4939-7225-8

Restricted until

2099-12-31