Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone
dc.contributor.author | Lin, Jane | |
dc.contributor.author | Mitchell, Naomi | |
dc.contributor.author | Kalcina, Marina | |
dc.contributor.author | Tchoubrieva, E | |
dc.contributor.author | Stewart, Mary | |
dc.contributor.author | Marygold, Steven | |
dc.contributor.author | Walker, Cherryl | |
dc.contributor.author | Thomas, George | |
dc.contributor.author | Leevers, Sally | |
dc.contributor.author | Pearson, Richard B | |
dc.contributor.author | Quinn, Leonie | |
dc.contributor.author | Hannan, Ross | |
dc.date.accessioned | 2018-11-29T22:55:32Z | |
dc.date.available | 2018-11-29T22:55:32Z | |
dc.date.issued | 2011 | |
dc.date.updated | 2018-11-29T08:06:54Z | |
dc.description.abstract | The ribosome is critical for all aspects of cell growth due to its essential role in protein synthesis. Paradoxically, many Ribosomal proteins (Rps) act as tumour suppressors in Drosophila and vertebrates. To examine how reductions in Rps could lead to tissue overgrowth, we took advantage of the observation that an RpS6 mutant dominantly suppresses the small rough eye phenotype in a cyclin E hypomorphic mutant (cycE JP). We demonstrated that the suppression of cycE JP by the RpS6 mutant is not a consequence of restoring CycE protein levels or activity in the eye imaginal tissue. Rather, the use of UAS-RpS6 RNAi transgenics revealed that the suppression of cycE JP is exerted via a mechanism extrinsic to the eye, whereby reduced Rp levels in the prothoracic gland decreases the activity of ecdysone, the steroid hormone, delaying developmental timing and hence allowing time for tissue and organ overgrowth. These data provide for the first time a rationale to explain the counter-intuitive organ overgrowth phenotypes observed for certain members of the Minute class of Drosophila Rp mutants. They also demonstrate how Rp mutants can affect growth and development cell non-autonomously. | |
dc.format.mimetype | application/pdf | en_AU |
dc.identifier.issn | 1553-7390 | |
dc.identifier.uri | http://hdl.handle.net/1885/153191 | |
dc.publisher | Public Library of Science | |
dc.source | PLoS Genetics | |
dc.subject | Keywords: cyclin E; ecdysone; mutant protein; ribosome protein; cyclin E; ecdysone; protein S6; animal tissue; article; cell proliferation; controlled study; Drosophila; female; growth regulation; insect development; nonhuman; phenotype; protein function; RNA inter | |
dc.title | Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone | |
dc.type | Journal article | |
dcterms.accessRights | Open Access | en_AU |
local.bibliographicCitation.issue | 12 | |
local.bibliographicCitation.lastpage | e1002408 | |
local.bibliographicCitation.startpage | e1002408 | |
local.contributor.affiliation | Lin, Jane, Peter MacCallum Cancer Centre | |
local.contributor.affiliation | Mitchell, Naomi, University of Melbourne | |
local.contributor.affiliation | Kalcina, Marina, Department of Anatomy and Cell Biology, University of Melbourne, | |
local.contributor.affiliation | Tchoubrieva, E, College of Science, ANU | |
local.contributor.affiliation | Stewart, Mary, Department of Biological Sciences, North Dakota State University, Fargo, North Dakota | |
local.contributor.affiliation | Marygold, Steven, Growth Regulation Laboratory, Cancer Research UK London Research Institute, London, United Kingdom | |
local.contributor.affiliation | Walker, Cherryl, Growth Regulation Laboratory, Cancer Research UK London Research Institute, London, United Kingdom | |
local.contributor.affiliation | Thomas, George, University of Cincinnati Medical Center, Cincinnati, Ohio, United States of America, | |
local.contributor.affiliation | Leevers, Sally, Growth Regulation Laboratory, Cancer Research UK London Research Institute, London, United Kingdom | |
local.contributor.affiliation | Pearson, Richard B, Peter MacCallum Cancer Centre | |
local.contributor.affiliation | Quinn, Leonie, College of Health and Medicine, ANU | |
local.contributor.affiliation | Hannan, Ross, College of Health and Medicine, ANU | |
local.contributor.authoremail | u9910136@anu.edu.au | |
local.contributor.authoruid | Tchoubrieva, E, u9910136 | |
local.contributor.authoruid | Quinn, Leonie, u1037504 | |
local.contributor.authoruid | Hannan, Ross, u1000203 | |
local.description.notes | Imported from ARIES | |
local.identifier.absfor | 111201 - Cancer Cell Biology | |
local.identifier.absseo | 920102 - Cancer and Related Disorders | |
local.identifier.ariespublication | u1013515xPUB8 | |
local.identifier.citationvolume | 7 | |
local.identifier.doi | 10.1371/journal.pgen.1002408 | |
local.identifier.scopusID | 2-s2.0-84855289269 | |
local.identifier.thomsonID | 000299167900025 | |
local.identifier.uidSubmittedBy | u1013515 | |
local.type.status | Published Version |
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