NDH-1 Is Important for Photosystem I Function of Synechocystis sp. Strain PCC 6803 under Environmental Stress Conditions
Date
2018
Authors
Zhao, Jiaohong
Gao, Fudan
Fan, Da Yong
Chow, Wah S
Ma, Weimin
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Research Foundation
Abstract
Cyanobacterial NDH-1 interacts with photosystem I (PSI) to form an NDH-1-PSI
supercomplex. Here, we observed that absence of NDH-1 had little, if any, effect on
the functional fractions of PSI under growth conditions, but significantly reduced the
functional fractions of PSI when cells of Synechocystis sp. strain PCC 6803 were
moved to conditions of multiple stresses. The significant reduction in NDH-1-dependent
functional fraction of PSI was initiated after PSII activity was impaired. This finding is
consistent with our observation that the functional fraction of PSI under growth conditions
was rapidly and significantly decreased with increasing concentrations of DCMU, which
rapidly and significantly suppressed PSII activity by blocking the transfer of electrons
from QA to QB in the PSII reaction center. Furthermore, absence of NDH-1 resulted in
the PSI limitation at the functionality of PSI itself but not its donor-side and acceptor-side
under conditions of multiple stresses. This was supported by the result of a significant
destabilization of the PSI complex in the absence of NDH-1 but the presence of multiple
stresses. Based on the above results, we propose that NDH-1 is important for PSI
function of Synechocystis sp. strain PCC 6803 mainly via maintaining stabilization of
PSI under conditions of environmental stresses.
Description
Keywords
Citation
Collections
Source
Frontiers in Plant Science
Type
Journal article
Book Title
Entity type
Access Statement
Open Access
License Rights
Creative Commons Attribution License (CC BY)
Restricted until
Downloads
File
Description