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A systematic study of labelling an α-helix in a protein with a lanthanide using IDA-SH or NTA-SH tags

Date

Authors

Yagi, Hiromasa

Maleckis, Ansis

Otting, Gottfried

Publisher

Kluwer Academic Publishers

Abstract

The previously published IDA-SH and NTA-SH tags are small synthetic lanthanide-binding tags derived from cysteine, which afford site-specific lanthanide labelling by disulfide-bond formation with a cysteine residue of the target protein. Following attachm

Keywords

Keywords: aspartic acid; cysteine; diglycine; glutamic acid; lanthanide; mutant protein; nitrilotriacetic acid; alpha helix; amino acid sequence; anisotropy; article; binding site; carbon nuclear magnetic resonance; chemical binding; chemical labeling; controlled s ERp29; IDA-SH tag; Lanthanide tag; NMR spectroscopy; NTA-SH tag; Pseudocontact shifts

URI

http://hdl.handle.net/1885/61737

Collections

ANU Research Publications

Source

Journal of Biomolecular NMR

Type

Journal article

DOI

10.1007/s10858-012-9697-3

Restricted until

2037-12-31

Downloads

File

Description

01_Yagi_A_systematic_study_of_2013.pdf (1009.05 KB)