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Frozen in time: a new method using cryo-scanning electron microscopy to visualize root-fungal interactions

Date

Authors

Refshauge, Stephen Johnson

Watt, Michelle

McCully, Margaret E

Huang, Cheng

Publisher

Cambridge University Press

Abstract

• A new method of sample preparation for cryo-scanning electron microscopy was used to visualize internal infection of wheat (Triticum aestivum) roots by the pathogenic fungus Rhizoctonia solani AG-8. The new method retained fungal hyphae and root cells

Keywords

Keywords: disease severity; electron microscopy; fungal disease; in situ test; wheat; article; cryoelectron microscopy; Hyphomycetes; methodology; microbiology; physiology; plant disease; plant root; scanning electron microscopy; ultrastructure; wheat; Cryoelectron Cryo-fixation; Cryo-planing; Cryo-scanning electron microscopy; Deep etching; Pathogen; Rhizoctonia solani; Root rot; Root-fungal interaction

URI

http://hdl.handle.net/1885/25332

Collections

ANU Research Publications

Source

New Phytologist

Type

Journal article

DOI

10.1111/j.1469-8137.2006.01825.x

Restricted until

2037-12-31

Downloads

File

Description

01_Refshauge_Frozen_in_time:_a_new_method_2006.pdf (294.66 KB)

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