Cultural advice

The Australian National University acknowledges, celebrates and pays our respects to the Ngunnawal and Ngambri people of the Canberra region and to all First Nations Australians on whose traditional lands we meet and work, and whose cultures are among the oldest continuing cultures in human history.

Aboriginal and Torres Strait Islander peoples are advised that ANU Library collections may include images, names, voices, and other representations of deceased persons.

Material in the collection may contain terms, language or views that reflect the period in which the item was created and may be considered inappropriate today.

Differentiation of Embryonic Stem Cells into Functional Neurons In Vitro

Simple item page
dc.contributor.authorBaharvand, Hossein
dc.contributor.authorPower, John
dc.contributor.authorOzsarac, Nesrin
dc.contributor.authorMatthaei, Klaus
dc.date.accessioned2015-12-13T23:07:37Z
dc.date.issued2004
dc.date.updated2015-12-12T08:09:59Z
dc.description.abstractEmbryonic stem (ES) cells are pluripotent cells that are derived from the inner cell mass of blastocysts. We produced neuronal cells by directed differentiation of a newly established murine ES cell line in vitro (Royan B1). Immunohistochemistry, scanning electron microscopy, Campenot's assay and electrophysiology were used to evaluate the differentiated neurons. Antibodies against microtubule-associated protein (MAP2), glutamic acid decarboxylase (GAD) and tyrosine hydroxylase (TH) antigens showed that the cells had GABAergic and dopaminergic characteristics. The Campenot's assay revealed there were a number of peripheral nerveous system related neurons but they were rare. The cells also produced action potentials and responded to electrical stimulation by expressing calcium channels in the processes. We showed therefore that functional neurons can be generated by directed differentiation from embryonic stem cells in vitro.
dc.identifier.issn0893-6609
dc.identifier.urihttp://hdl.handle.net/1885/86288
dc.publisherJohn Wiley & Sons Inc
dc.sourceNeuroscience Research Communications
dc.titleDifferentiation of Embryonic Stem Cells into Functional Neurons In Vitro
dc.typeJournal article
local.bibliographicCitation.issue2
local.bibliographicCitation.lastpage138
local.bibliographicCitation.startpage130
local.contributor.affiliationBaharvand, Hossein, Royan Institute Tehran
local.contributor.affiliationPower, John, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOzsarac, Nesrin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidPower, John, u9909211
local.contributor.authoruidOzsarac, Nesrin, u9912080
local.contributor.authoruidMatthaei, Klaus, u8200697
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060410 - Neurogenetics
local.identifier.ariespublicationMigratedxPub15114
local.identifier.citationvolume35
local.identifier.doi10.1002/nrc.20027
local.identifier.scopusID2-s2.0-9144239853
local.type.statusPublished Version

Downloads

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
01_Baharvand_Differentiation_of_Embryonic_2004.pdf
Size:
1.35 MB
Format:
Adobe Portable Document Format
Download

Collections

ANU Research Publications

DSpace software copyright © 2002-2026 LYRASIS