The Glycine max Xylem Sap and Apoplast Proteome

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dc.contributor.authorDjordjevic, Michael
dc.contributor.authorOakes, Marie
dc.contributor.authorLi, Dong Xue
dc.contributor.authorHwang, Cheol Ho
dc.contributor.authorHocart, Charles
dc.contributor.authorGresshoff, Peter
dc.date.accessioned2015-12-10T22:32:17Z
dc.date.issued2007
dc.date.updated2015-12-09T10:15:03Z
dc.description.abstractMolecular signaling interactions in the plant apoplast are important for defense and developmental responses. We examined the soybean proteome of the apoplastic conduit of root-to-shoot communication, the xylem stream, using gel electrophoresis combined with two types of tandem mass spectrometry. We examined soybeans for the presence of a Bradyrhizobium japonicum-induced, long distance developmental signal that controls autoregulation of nodulation (AON) to determine if xylem proteins (XPs) were involved directly or indirectly in AON. The xylem and apoplast fluids collected in hypocotyl, epicotyl, and stem tissue contained a highly similar set of secreted proteins. The XPs were different from those secreted from imbibing seed implying they play important basic roles in xylem function. The XPs of wild-type and nts1007 plants were indistinguishable irrespective of plant age, inoculation status, or time after inoculation suggesting that none was directly involved in AON. XPs were continuously loaded into the xylem stream, as they were present even 28 h after shoot decapitation. These results were consistent with semiquantitative RT-PCR studies that examined the expression of genes corresponding to the XPs under inoculated or uninoculated conditions. Monitoring the expression of XP genes by RT-PCR showed that four possessed root biased expression. This suggested that the corresponding protein products could be produced in roots and travel long distances to shoots. Of these, a species of lipid transfer protein is a candidate for a water-soluble, long-distance signal-carrier due to the presence of hydrophobic clefts that bind known plant signals in vitro. Two soybean XPs identified in this study, lipid transfer protein and Kunitz trypsin inhibitor (KTI), have known roles in plant signaling.
dc.identifier.issn1535-3893
dc.identifier.urihttp://hdl.handle.net/1885/55686
dc.publisherAmerican Chemical Society
dc.sourceJournal of Proteome Research
dc.subjectKeywords: soybean protein; vegetable protein; apoplast; article; autoregulation; Bradyrhizobium japonicum; gel electrophoresis; hydrophobicity; inoculation; matrix assisted laser desorption ionization time of flight mass spectrometry; nodulation; nonhuman; plant ge Autoregulation-of-nodulation; Bradyrhizobium japonicum; Lipid transfer proteins; MALDI-TOF/TOF; Model legume; Root-to-shoot signalling
dc.titleThe Glycine max Xylem Sap and Apoplast Proteome
dc.typeJournal article
local.bibliographicCitation.lastpage3779
local.bibliographicCitation.startpage3771
local.contributor.affiliationDjordjevic, Michael, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOakes, Marie, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLi, Dong Xue, University of Queensland
local.contributor.affiliationHwang, Cheol Ho, University of Queensland
local.contributor.affiliationHocart, Charles, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGresshoff, Peter, University of Queensland
local.contributor.authoremailu8400044@anu.edu.au
local.contributor.authoruidDjordjevic, Michael, u8400044
local.contributor.authoruidOakes, Marie, u1533595
local.contributor.authoruidHocart, Charles, u8101127
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu9204316xPUB336
local.identifier.citationvolume6
local.identifier.doi10.1021/pr0606833
local.identifier.scopusID2-s2.0-34948818726
local.identifier.uidSubmittedByu9204316
local.type.statusPublished Version

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