A unique H2A histone variant occupies the transcriptional start site of active genes

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dc.contributor.authorSoboleva, Tatiana
dc.contributor.authorNekrasov, Maxim
dc.contributor.authorPahwa, Anuj
dc.contributor.authorWilliams, Rohan
dc.contributor.authorHuttley, Gavin Austin
dc.contributor.authorTremethick, David
dc.date.accessioned2015-12-10T22:58:12Z
dc.date.issued2012
dc.date.updated2016-02-24T09:27:06Z
dc.description.abstractTranscriptional activation is controlled by chromatin, which needs to be unfolded and remodeled to ensure access to the transcription start site (TSS). However, the mechanisms that yield such an 'open' chromatin structure, and how these processes are coordinately regulated during differentiation, are poorly understood. We identify the mouse (Mus musculus) H2A histone variant H2A.Lap1 as a previously undescribed component of the TSS of active genes expressed during specific stages of spermatogenesis. This unique chromatin landscape also includes a second histone variant, H2A.Z. In the later stages of round spermatid development, H2A.Lap1 dynamically loads onto the inactive X chromosome, enabling the transcriptional activation of previously repressed genes. Mechanistically, we show that H2A.Lap1 imparts unique unfolding properties to chromatin. We therefore propose that H2A.Lap1 coordinately regulates gene expression by directly opening the chromatin structure of the TSS at genes regulated during spermatogenesis.
dc.identifier.issn1545-9993
dc.identifier.urihttp://hdl.handle.net/1885/60754
dc.publisherNature Publishing Group
dc.sourceNature Structural and Molecular Biology
dc.subjectKeywords: histone H2A; article; chromatin structure; gene expression; genetic transcription; genetic variability; human; molecular mechanics; priority journal; spermatid; spermatogenesis; transcription initiation; X chromosome; Amino Acid Sequence; Animals; Blottin
dc.titleA unique H2A histone variant occupies the transcriptional start site of active genes
dc.typeJournal article
local.bibliographicCitation.issue1
local.bibliographicCitation.lastpage31
local.bibliographicCitation.startpage25
local.contributor.affiliationSoboleva, Tatiana, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationNekrasov, Maxim, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPahwa, Anuj, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWilliams, Rohan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHuttley, Gavin Austin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTremethick, David, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu9900301@anu.edu.au
local.contributor.authoruidSoboleva, Tatiana, u9900301
local.contributor.authoruidNekrasov, Maxim, u4610325
local.contributor.authoruidPahwa, Anuj, u4617066
local.contributor.authoruidWilliams, Rohan, u4464804
local.contributor.authoruidHuttley, Gavin Austin, u9800703
local.contributor.authoruidTremethick, David, u9100316
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060404 - Epigenetics (incl. Genome Methylation and Epigenomics)
local.identifier.absfor060405 - Gene Expression (incl. Microarray and other genome-wide approaches)
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.ariespublicationf5625xPUB561
local.identifier.citationvolume19
local.identifier.doi10.1038/nsmb.2161
local.identifier.scopusID2-s2.0-84855457949
local.identifier.thomsonID000299046000006
local.identifier.uidSubmittedByf5625
local.type.statusPublished Version

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