Cultural advice

The Australian National University acknowledges, celebrates and pays our respects to the Ngunnawal and Ngambri people of the Canberra region and to all First Nations Australians on whose traditional lands we meet and work, and whose cultures are among the oldest continuing cultures in human history.

Aboriginal and Torres Strait Islander peoples are advised that ANU Library collections may include images, names, voices, and other representations of deceased persons.

Material in the collection may contain terms, language or views that reflect the period in which the item was created and may be considered inappropriate today.

Hypothesis: A biological role for germline transcription in the mechanism of V(D)J recombination - implications for initiation of allelic exclusion

Simple item page
dc.contributor.authorFranklin, Andrew
dc.date.accessioned2015-12-13T23:03:45Z
dc.date.issued2006
dc.date.updated2015-12-12T07:50:59Z
dc.description.abstractThe sequences that encode the antigen-binding sites of IgH and IgL chains - variable (V), diversity (D, H chain loci only) and joining (J) sequences - are configured as separate DNA segments at the germline level. Expression of an Ig molecule requires V(D)J assembly. Productive V(D)J recombination is monoallelic. How rearrangement is initiated differentially at maternal and paternal alleles is unclear. The products of recombination activating gene (RAG)1 and RAG2 mediate rearrangement by cleaving the DNA between an unrearranged gene segment and adjacent recombination signal sequences (RSS). It is proposed that supercoiling generated during germline transcription at Ig loci (which occurs concomitantly with rearrangement) is required at RSS for RAG1/2 recognition. Rearrangement might hence initiate sequentially at maternal and paternal alleles where deregulated germline transcription causes RAG1/2 recognition of RSS to become stochastic.
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/1885/85063
dc.publisherBlackwell Publishing Ltd
dc.sourceImmunology and Cell Biology
dc.subjectKeywords: DNA; immunoglobulin heavy chain; immunoglobulin light chain; RAG1 protein; RAG2 protein; allele; animal cell; article; B lymphocyte; controlled study; DNA determination; gene expression; gene sequence; genetic code; genetic transcription; genetic variabil Allelic exclusion; Germline transcription; Recombination activating gene; Recombination signal sequence; V(D)J recombination
dc.titleHypothesis: A biological role for germline transcription in the mechanism of V(D)J recombination - implications for initiation of allelic exclusion
dc.typeJournal article
local.bibliographicCitation.lastpage403
local.bibliographicCitation.startpage396
local.contributor.affiliationFranklin, Andrew, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidFranklin, Andrew, u9913738
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060406 - Genetic Immunology
local.identifier.ariespublicationMigratedxPub13263
local.identifier.citationvolume84
local.identifier.doi10.1111/j.1440-1711.2006.01437.x
local.identifier.scopusID2-s2.0-33745079671
local.type.statusPublished Version

Downloads

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
01_Franklin_Hypothesis:_A_biological_role_2006.pdf
Size:
165.11 KB
Format:
Adobe Portable Document Format
Download

Collections

ANU Research Publications

DSpace software copyright © 2002-2026 LYRASIS