Partially Dissecting Electron Fluxes in Both Photosystems in Spinach Leaf Disks during Photosynthetic Induction

dc.contributor.authorZhang, Meng-Meng
dc.contributor.authorFan, Da Yong
dc.contributor.authorMurakami, Keach
dc.contributor.authorBadger, Murray
dc.contributor.authorSun, Guang-Yu
dc.contributor.authorChow, Wah Soon
dc.date.accessioned2020-06-10T23:57:25Z
dc.date.issued2019
dc.date.updated2019-12-19T07:44:54Z
dc.description.abstractPhotosynthetic induction, a gradual increase in photosynthetic rate on a transition from darkness or low light to high light, has ecological significance, impact on biomass accumulation in fluctuating light and relevance to photoprotection in strong light. However, the experimental quantification of the component electron fluxes in and around both photosystems during induction has been rare. Combining optimized chlorophyll fluorescence, the redox kinetics of P700 [primary electron donor in Photosystem I (PSI)] and membrane inlet mass spectrometry in the absence/presence of inhibitors/mediator, we partially estimated the components of electron fluxes in spinach leaf disks on transition from darkness to 1,000 mmol photonsm2 s 1 for up to 10 min, obtaining the following findings: (i) the partitioning of energy between both photosystems did not change noticeably; (ii) in Photosystem II (PSII), the combined cyclic electron flow (CEF2) and charge recombination (CR2) to the ground state decreased gradually toward 0 in steady state; (iii) oxygen reduction by electrons from PSII, partly bypassing PSI, was small but measurable; (iv) cyclic electron flow around PSI (CEF1) peaked before becoming somewhat steady; (v) peak magnitudes of some of the electron fluxes, all probably photoprotective, were in the descending order: CEF1 > CEF2 +CR2 > chloroplast O2 uptake; and (vi) the chloroplast NADH dehydrogenase-like complex appeared to aid the antimycin A-sensitive CEF1. The results are important for fine-tuning in silico simulation of in vivo photosynthetic electron transport processes; such simulation is, in turn, necessary to probe partial processes in a complex network of interactions in response to environmental changes.
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn0032-0781en_AU
dc.identifier.urihttp://hdl.handle.net/1885/204930
dc.language.isoen_AUen_AU
dc.publisherJapanese Society of Plant Physiologistsen_AU
dc.relationhttp://purl.org/au-research/grants/arc/CE140100015en_AU
dc.rights© The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologistsen_AU
dc.sourcePlant and Cell Physiologyen_AU
dc.titlePartially Dissecting Electron Fluxes in Both Photosystems in Spinach Leaf Disks during Photosynthetic Inductionen_AU
dc.typeJournal articleen_AU
local.bibliographicCitation.issue10en_AU
local.bibliographicCitation.lastpage2219en_AU
local.bibliographicCitation.startpage2206en_AU
local.contributor.affiliationZhang, Meng-Meng, College of Science, ANUen_AU
local.contributor.affiliationFan, Da Yong, College of Science, ANUen_AU
local.contributor.affiliationMurakami, Keach, College of Science, ANUen_AU
local.contributor.affiliationBadger, Murray, College of Science, ANUen_AU
local.contributor.affiliationSun, Guang-Yu, Northeast Forestry Universityen_AU
local.contributor.affiliationChow, Wah S (Fred), College of Science, ANUen_AU
local.contributor.authoremailu8002735@anu.edu.auen_AU
local.contributor.authoruidZhang, Meng-Meng, u1030927en_AU
local.contributor.authoruidFan, Da Yong, u1812991en_AU
local.contributor.authoruidMurakami, Keach, u1052265en_AU
local.contributor.authoruidBadger, Murray, u8002735en_AU
local.contributor.authoruidChow, Wah S (Fred), u9609696en_AU
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060705 - Plant Physiologyen_AU
local.identifier.absseo820215 - Vegetablesen_AU
local.identifier.ariespublicationu5786633xPUB1100en_AU
local.identifier.citationvolume60en_AU
local.identifier.doi10.1093/pcp/pcz114en_AU
local.identifier.scopusID2-s2.0-85073183680
local.identifier.uidSubmittedByu5786633en_AU
local.publisher.urlhttp://www.oxfordjournals.org/en_AU
local.type.statusPublished Versionen_AU

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